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KMID : 0545120000100010021
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.21 ~ p.26
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Development of DNA Chip Microarrayer
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Yoon, Sung Ho
Choi, Jong Gil/Lee, Sang Yup
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Abstract
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A microarrayer system was developed mainly for manufacturing DNA chips. The 3-axis robot was designed to automatically collect samples from 96-or 384-well microtiter plates using up to 16 simultaneously moving pens and to deposit them on a surface-modified slide glass. This is followed by a wash/dry operation in a clean station. The cycle is repeated with a new set of samples. This system can deposit cDNA or oligonucleotides with spot intervals of 150㎛ and the spot size of 80㎛, thus allowing a high density DNA chip containing about 5,000 spots per ㎠. The entire procedure is controlled by the Visual C++ program that was written in our laboratory by using a personal computer with Pentium 100 CPU.
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